Enzymatic Sculpting of Nanoscale and Microscale Surface Topographies

Angewandte Chemie International Edition

Volume 51, Issue 38,pages 9619–9623,September 17, 2012

Lithography-free etching of complex surface features is achieved by harnessing the enzyme proteinase K (PK), controlled by bovine serum albumin (BSA), to digest a biodegradable polymer. This bio-sculpting process is used to construct a membraneless filtration device for the size-based isolation and enrichment of cells from whole blood.