Center for Fundamental Living Technology (FLinT), Institute of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Yamadaoka 1-5, Suita, Osaka 565-0871
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
Department of Bioinformatic Engineering,
Graduate School of Information Science and Technology, and Department of Frontier Biosciences,
Graduate School of Frontier Biosciences, Osaka University, Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
The membrane properties of phospholipid vesicles can be manipulated to both regulate and initiate encapsulated biochemical reactions and networks. We present evidence for the inhibition and activation of reactions encapsulated in vesicles by the exogenous addition of charged amphiphiles. While the incorporation of cationic amphiphile exerts an inhibitory effect, complementation of additional anionic amphiphiles revitalize the reaction. We demonstrated both the simple hydrolysis reaction of β-glucuronidase and the in vitro gene expression of this enzyme from a DNA template. Furthermore, we show that two vesicle populations decorated separately with positive and negative amphiphiles can fuse selectively to supply feeding components to initiate encapsulated reactions. This mechanism could be one of the rudimentary but effective means to regulate and maintain metabolism in dynamic artificial cell models.
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