Rapid, Sensitive, and Quantitative Detection of Pathogenic DNA at the Point of Care through Microfluidic Electrochemical Quantitative Loop-Mediated Isothermal Amplification†

1. Kuangwen Hsieh^2,‡, 
2. Adriana S. Patterson^3,‡, 
3. Dr. B. Scott Ferguson², 
4. Prof. Kevin W. Plaxco³, 
5. Prof. H. Tom Soh^1,2,

1. Article first published online: 4 APR 2012
   DOI: 10.1002/anie.201109115
   Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
   
   ingle-step DNA detection: A microfluidic electrochemical loop-mediated isothermal amplification platform is reported for rapid, sensitive, and quantitative detection of pathogen genomic DNA at the point of care (see picture). DNA amplification was electrochemically monitored in real time within a monolithic microfluidic device, enabling the detection of as few as 16 copies of Salmonella genomic DNA through a single-step process in less than an hour.