Live 3D cell imaging

Live-cell 3D super-resolution imaging in thick biological samples

• Francesca Cella Zanacchi,
• Zeno Lavagnino,
• Michela Perrone Donnorso,
• Alessio Del Bue,
• Laura Furia,
• Mario Faretta
• & Alberto Diaspro

• Nature Methods
   
  (2011)
   
  doi:10.1038/nmeth.1744

• We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50–150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.